Can the Non strand specify RNAseq data annotate genome?

Question

[yiren]

Hi everyone,

I have many rnaseq samples data, but I find those data are not strand specific. I read many paper and I found they annotated their genome using the strand specific RNAseq data. Can I use my non-strand specify RNAseq data to annotate gene structure of genome?Thank you very much.

Answer 1

[Juke34]

Yes sure you can, it is just most of time more accurate to perform annotation using stranded library RNAseq data.

[yiren]

thank you very much.