What is the difference between filtering cells before creating Seurat object and after that?

Question

[re_raz]

I tried filter cells that have unique feature counts over 5000 or less than 350 and > 2% mitochondrial counts before creating Seurat object, and I also tried to do this step after creating Seurat object. The results looked the same. Is there any difference between the two ways that I might not figure out? Which way is the best ?

[rpolicastro]

Both methods are fine.

[re_raz]

Thank you