Input source for the IPA tool

Question

[Mozart]

Dear all, Just wondering if people generally import full list of genes from an RNA-seq experiment or just the list of genes that reached significance as the input file for the Ingenuity Pathway Analysis tool? Thanks.

Answer 1

[GenoMax]

Up to you as the user. You could bring in extended data and then filter inside IPA so you always have the full data available. Or you could simply start with a list of genes you have pre-selected elsewhere by criteria you had decided (fold-changes etc).

[Mozart]

Thanks a lot for confirming this. This is really reassuring!