How to use "SingleR" on the marker genes from `FindAllMarkers` for each cluster?
0
1
Entering edit mode
4.0 years ago

Hi,

I tried to use SingleR to identify cell types for clusters. I have the table of results from FindAllMakers of Seurat package.

I know that I can use:

SingleR(GetAssayData(seurat.object, 
        assay = assay, slot = "data"), clusters = Idents(seurat.object),
        ref = hpca.se, labels = hpca.se$label.main)

But is there any way I can utilize the markers for each cluster to define cell types using SingleR ?

Thank you for help.

SingleR seurat scRNAseq scRNA • 4.1k views
ADD COMMENT
0
Entering edit mode

I am also looking for an answer regarding this issue. Any update?

ADD REPLY
0
Entering edit mode

My problem is similar: I would like to use/compare markers from my dataset and markers from a reference (with labels) to annotate my dataset.

ADD REPLY

Login before adding your answer.

Traffic: 1809 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6