I have tones of FAST5 files from MiniON (obtained from intestines of ticks) and my goal is to analyse the species composition (microbiome analysis). We used 16S barcoding kit for Min106 flowcell. Can anyone help me to understand what tools to use step by step to perform it except EPI2ME.
There are many ways to accomplish this goal; however, it will depend on the type of data you generated. Sometimes people use the term
metagenomics
to mean different things, e.g., targeted sequencing of an amplicon or untargeted sequencing of all genetic content. Can you edit your question to clarify the type of data you have (e.g., 16S/18S or shotgun metagenomic data)?