Beginner in bioinformatics needs some help

Question

[Monika]

Hi, I am new in Bioinformatics, trying to analyze two colour agilent microarray. I have decided to follow this protocol build the expression matrix step by step from GEO raw data I have created a folder that i have named "Targets.txt". Inside I have created folder "SampleFiles" and downstream three subfolders "Array1", "Array2" and "Array3". I want to read those files and I get a message PErmission denied. I know it is super simple but please can anyone help me.

[ATpoint]

Hard to answer, we currently have no idea from which data and from which format you start. CEL files? A matrix with intensity values? Some commands would help that generated this error.

[Monika]

My files are in the format: 8M-8 SG11410002_257236320035_S002_GE2_107_Sep09_2_4 I have 8 files in .txt format per array. 3 arrays in total.

[Hyper_Odin]

Where are you trying to open this file ? R-studio?

[Monika]

Yep, R Studio.

targetinfo <- readTargets('Targets.txt', sep = '\t') Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") : cannot open file 'Targets.txt': Permission denied

[Hyper_Odin]

Right click on R-studio and run it as administrator. Hopefully this should solve the problem.

[Monika]

I solved the problem :) Thank you :)