non-unique values when setting 'row.names' error in DESeq2

Question

[Sai Prasada]

Hi Everyone!

I am trying to run the DESeqDataSetFromMatrix() command using a specific dataset but I keep getting the following error.

For some reason, R (version 4.0.5) is trying to assign the first column of my DataFrame to be the row names, even though I have already assigned the row names to be the gene names.

Also, here is a sampling of the data that I'm working with.

I am currently working in R version 4.0.5. Any help or pointers in the right direction would be greatly appreciated :)

[cpad0112]

can you try without tidy=TRUE ?

Answer 1

[Basti]

Remove tidy=TRUE, because tidy option is "for matrix input: whether the first column of countData is the rownames for the count matrix"

[Sai Prasada]

Yes! This worked thank you so much!