Exome sequencing one sample on two lanes considered two read groups for marking of PCR duplicates?

Question

[rebeliscu]

I'm asking because I don't know whether to mark different lanes as a different library for the purpose of marking PCR duplicates. My instinct is that different lanes from the same library should still be treated as a single read group, since PCR duplicates could be found across lanes, no?

Thanks in advance.

[GenoMax]

If it is the same library that run on two lanes then it is a technical sequencing replicate.

[rebeliscu]

Sorry, I'm somewhat new to all of this. Does that imply PCR duplicate and thus the presence of duplicate reads should be considered across lanes?

[GenoMax]

Yes because it is the same library running on different lanes to get more data.