Is there any reason to discard singletons after de-hosting metagenomic reads?
0
0
Entering edit mode
3.1 years ago

I an double de hosting metagenomic reads and am wondering if there is any reason to discard singletons after both alignment steps? The workflow is as:

  1. Map to host 1
  2. Convert to fastq and repair.sh to resort
  3. Map to host 2
  4. Convert to fastq and repair.sh to resort
  5. Assemble

During steps 2 and 4 I believe the default behaviour of repair.sh is to discard singletons but I have the option to output them separately. Is there any reason not to recombine the singletons with the origin fastq file before assembly (step 5)?

alignment metagenome • 563 views
ADD COMMENT

Login before adding your answer.

Traffic: 1965 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6