Hi All,
I am new to the scRNA_seq data analysis. Recently I analyzed a treatment vs control scRNA-seq. Treatment group has samples treated with a compound, which has shown positive effect (reduction in disease). Purpose of the study is to understand the mechanism of action of compounds. I used seurat for data analysis and identified some biomarker genes within cell types which are most differentially expressed between the two groups. After doing pathway analysis I would now like to find gene regulatory networks in order to find which genes are regulators and understand mechanism. Has anyone done similar kind of analysis? Looking for tools/packages to find GRN? Any thoughts or suggestion will be appreciated.