FeatureCount summary in RNA_Seq analysis

Question

[Diango]

Hi, I have a doubt about the number of reads mapped to genes in the featureCounts report. Is it possible that the number of reads mapped to genes is greater than the total number of reads?

For exemple :

Mapping repport : using STAR input reads = 20 000 000

FeatureCounts repport : Reads mapped to genes (Assigned) : 42 000 000

[swbarnes2]

Since you seem to have done it, it's probably possible. If you want someone to troubleshoot your process, you'll have to actually tell people what your command lines were.

[Diango]

Thanks swbarnes2 for your quick response, of course I did. I can't figure out why the number of reads mapped to genes is greater than the total number of reads mapped to reference genome ? Is it normal or not? Thank you