Multiple sequence data from same DNA sample

Question

[Hirofumi]

Hello. I want to detect rare variant without sequence error. However, average estimated-sequence error is 0.3%. So, I am going to do multiple sequence (4 times) from same DNA sample.

But, I don't know how to marge and analyze from these data (.fastq). Please teach me any idea.

Thank you for reading!

Answer 1

[Joe]

If they are from the same sample, sequenced in the same way, you can literally just concatenate the FASTQ files together (e.g. with cat).

Depending on how much sequencing you're doing, and the organism, you may end up with too much data and need to randomly downsample this if you intend on doing any genome assembly.

[Hirofumi]

I see! Thank you. I'll try it!