Using IndexFeatureFile to index vcf
1
1
Entering edit mode
3.0 years ago
shpak.max ▴ 50

I have a number of vcfs that I need to index (create .idx files), which I attempted to do with GATK, e.g.

java -Xmx5g -jar $gatk_path/GenomeAnalysisTK.jar -T IndexFeatureFile -F N03_INDELS.vcf 

Which returns an invalid argument error even though the vcf file is, as far as I can tell, in standard format.

##### ERROR MESSAGE: Invalid command line: Malformed walker argument: Could not find    walker with name: IndexFeatureFile

I haven't used IndexFeatureFile before (usually the idx is automatically generated when I create vcfs from my alignments using GATK), so it isn't clear to me what's going wrong.

GATK • 2.7k views
ADD COMMENT
1
Entering edit mode
3.0 years ago

there no such tool in gatk3.8

 java -jar gatk-3.8/GenomeAnalysisTK.jar -h 2>&1 | grep Index

it looks like it exists in gatk4.* https://gatk.broadinstitute.org/hc/en-us/articles/360037428111-IndexFeatureFile

anyways, it's more 'standard' to work with '*.vcf.gz" files indexed with bcftools index -t -f in.vcf.gz

ADD COMMENT
0
Entering edit mode

Thanks. Is there anything analogous in GATK 3 that would create idx files?

ADD REPLY

Login before adding your answer.

Traffic: 1923 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6