Using simple t-test for selected genes of the microarray dataset

Question

[seta]

Dear all,

I've done a meta-analysis on the gene expression data of given disease. Now, I would like to see whether the some differential expressed genes will be also validated in another independent datasets. I'm thinking to select the genes of interest from independent microarry dateset and doing a simple t-test? Could you please let me know if this is a right approach? or is it better to analyse the whole independent dataset and look for the genes of interest among the results?

Best

[seta]

Many thanks, ATpoint. Strange, your comment has not appeared here, but in my messages box. The data used for meta-analysis is Microarray, not RNA-seq. I go for analyzing the entire dataset as you suggested.