Entering edit mode
3.0 years ago
mrashad
▴
80
Dears, Could you help me to understand the difference between the following tags of transcripts in Ensemble, please ? and which one of them would be used to design primers of qRT-PCR? I tried to understand them through this link: https://m.ensembl.org/info/genome/genebuild/transcript_quality_tags.html But couldn't understand the difference.
Page you linked above has detailed explanation of the flags. Which part do you need help with?
If you are simply looking for the best representative then using one marked
Ensembl canonical
would be your best bet.