How to find a list homologous genes from different species?
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2.9 years ago
WUSCHEL ▴ 810

I have around 100 of genes of interested of a biological pathway of a model organism Arabidopsis thaliana. How can i extract these genes / protein sequences at single step (automated) — rather doing one by one. And then I want to find their homologous genes in two other plant species (Pisum sativa and Oryza sativa). Could someone guide me how to do this— is there any R programming pipeline for such work or any online tools?

RNA-Seq R Proteomics • 1.4k views
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I note that OMA has an R API, see Expanding the Orthologous Matrix (OMA) programmatic interfaces: REST API and the OmaDB packages for R and Python.I don't know how easy it is to limit to certain species.

And this one isn't R,I don't think, yet seems to address your needs:
orthofisher: a broadly applicable tool for automated gene identification and retrieval (I note it has a tutorial, too!)
Github repo: https://github.com/JLSteenwyk/orthofisher

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Thank you very much Wayne. I will have a look if this helps

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2.9 years ago

if an online tool/platform is an option I can recommend PLAZA

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Thank you very much. I will have a look if this helps.

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