RNASeq Analysis — Galaxy Australia

Question

[WUSCHEL]

I am a biologist do not have very good exposure to bioinformatics.

Recently I came across some of my colleagues are using Galaxy Australia to analyze RNASeq data.

I had analyzed RNASeq data using R Programming Bioconductor packages (EdgeR, and DESEQ) along with FastQC, Trim_Galore, Kallisto. It was challenging to get access to servers and learn Linux.

May I know what is Galaxy Australia and how it has advantages over other pipelines?

I am interested in doing an RNASeq for a few crops which are not well-annotated genome.

Answer 1

[cpad0112]

I had analyzed RNASeq data using R Programming Bioconductor packages (EdgeR, and DESEQ) along with FastQC, Trim_Galore, Kallisto. It was challenging to get access to servers and learn Linux.

Most of these tools are available on Galaxy (probably on Australia Galaxy) and you can drag and drop the tools to construct a workflow. Once workflow is built, customize the parameters on UI and execute the workflow

May I know what is Galaxy Australia 

Galaxy is a web based, click and run data analysis platform to execute tools and workflows. Galaxy Australia is a server hosting Galaxy platform, maintained by this team based on Australia.

and how it has advantages over other pipelines?

Galaxy Australia or Galaxy it self is not a pipeline. It's a data analysis framework. Compared to other frameworks (esp NGS data analysis), learning curve is minimal with Galaxy server for creating, editing and executing workflows. It's advantages are drag and drop building of workflows, visual editing of workflows, sharing with users, export the workflow and history. In addition, user can search, import and execute workflows shared by community members within Galaxy.

[WUSCHEL]

Thank you cpad0112 — as always

Answer 2

[GenoMax]

Galaxy mirror in Australia : https://usegalaxy.org.au/

Galaxy FAQ: https://galaxyproject.org/support/

Dedicated help forum for Galaxy: https://help.galaxyproject.org/

[WUSCHEL]

Thank you very much GenoMax