Entering edit mode
2.9 years ago
Aaron
▴
30
Would someone be able to explain the difference between the "--solostrand" and "--readstrand" commands in STARsolo? What would it look like if I set each "Unstranded" vs e.g. "Forward"? I'm reading documentation for it (https://physiology.med.cornell.edu/faculty/skrabanek/lab/angsd/lecture_notes/STARmanual.pdf) but I'm not exactly clear about it. (This is my first time analyzing RNA-seq data though.)