List of cell cycle markers to use as reference in CellCycleScoring() in Seurat

Question

[Aaron]

I'm using Seurat to regress out cell cycle markers in some data that I have (with the function CellCycleScoring()). The data that I have was collected from Grcm38 mice but the closest available set of cycle markers I have been able to find are from Grcm39 mice (here: https://github.com/hbc/tinyatlas/tree/master/cell_cycle) - how much will these two things be functionally the same? I'm sure other people with Grcm38 data have used regressed out cell cycle markers from their data so better yet, is there a list of cell cycle markers from Grcm38 mice anywhere online?

[ATpoint]

Gene name is gene name. The genome version for these highly conserved genes does not matter I would feel save using those from the link.

Answer 1

[jared.andrews07]

There is an issue on the Seurat Github stating how to get around this.

[Aaron]

Thank you @jared.andrews07 you've really helped me a lot here!