Entering edit mode
2.8 years ago
Aaron
▴
30
Hi all, I'm trying to use Seurat's FindConservedMarkers() to find the conserved markers for a population I created with the CellSelector() function. It hasn't been working unfortunately, saying "Error: Subscript out of bounds" - I have tried the FindConservedMarkers() function on other clusters and it appears to work fine. After using CellSelector(), do I have to rerun FindNeighbors() or RunPCA() etc. in order to run FindConservedMarkers()? Is the error possibly because the cell cluster is too small? (It's about 250 cells.) Any other guidance would be much appreciated.