Ancient DNA Analysis Fastq to vcf

Question

[Aruna]

When combining multiple sample into vcf file, is it necessary that all samples must be aligned to the same reference build version ? such as Grch38, 37 etc.,

Answer 1

[tacrolimus]

Dear Aruna,

Yes! You will have to do a liftover from one build to another before merging into a single VCF

All the best