How to specify the depth of sequencing in ion torrent?

Question

[shersky]

I have never used ion torrent machine before, but in Illumina there is a calculator to specify the coverage before sequencing. Is there for ion torrent a similar thing, or in other words, can it be controlled in ion torrent sequencing ?

[Ram]

Do not delete posts that have received feedback. Interact with the people that have invested effort into helping you, and work towards providing closure to your post.

Answer 1

[Istvan Albert]

The coverage of a sequencing run is the size of the genome divided by the number of total bases sequenced.

When an instrument produces identical read lengths then the formula is

 coverage = number_of_reads * lenght_of_each_read / genome_size

The genome size is the expected length of the DNA that gets fragmented.