I am new to HHpred/ analyzing proteins so bare with me. I have been given an uncharacterized protein, whose FASTA sequence I have obtained from Uniprot. I am looking to do the following by using HHpred.
So far, I have done the following:
obtained FASTA sequence and inputted it into HHpred. Loaded the FASTA sequence on to HHpred. What I am looking to do on HHpred now:
Identify the different domains/motifs of this uncharacterized protein Compare full length protein to two separate domains Determine if the predictions has improved by comparing the full length proteins to the separate domains. All comments help, I am very desperate for any tips.
Thank you!
Biostars is a very nice site to look for something unknown. Your question has already had some similar posts on the right panel. Look through them. For example, this post shows you several ways to start with.
What Is The Concept Of Secondary Structure Prediction By Using Ncbi Blast, Hhpred And Jpred?