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2.8 years ago
I have with me 100 Ecoli strains and I would like to perform reference-free variant calling. I would like to use the De-Bruijn graph method to go about it. Which tool would be the best to go about it? I have gone through Cortex, which I feel shall help. But I am a little lost with how to begin. The manual is descriptive, but it has too much information and it is a little overwhelming for a beginner like me. Any suggestions in this regard will help :)
