How to use eprimer3 command in linux?
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2.8 years ago
Ahmad ▴ 10

Hallow, every one

when I use eprimer3 to generate primers for fasta sequence of sars cov 2 whole genome give Error:

Argument 'saracovII.fasta' : Too many parameters 3/2

what the best way to write eprimer3 command line to generate primers I used this command

eprimer3 -outfile=out.pr3 -sequence= saracovII.fasta

Thanks for your attention

ePrimer3 NCBI terminal • 828 views
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remove the blank after sequence=

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after remove the blank get new error

Pick PCR primers and hybridization oligos Error: Missing SEQUENCE tag

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2.8 years ago
Ahmad ▴ 10

I dissolve the problem

EMBOSS_PRIMER32_CORE=/usr/bin/primer3_core eprimer32 -sequence=saracovII.fasta -outfile=out.pr3
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