Entering edit mode
2.8 years ago
Ahmad
▴
10
Hallow, every one
when I use eprimer3 to generate primers for fasta sequence of sars cov 2 whole genome give Error:
Argument 'saracovII.fasta' : Too many parameters 3/2
what the best way to write eprimer3 command line to generate primers I used this command
eprimer3 -outfile=out.pr3 -sequence= saracovII.fasta
Thanks for your attention
remove the blank after
sequence=
after remove the blank get new error
Pick PCR primers and hybridization oligos Error: Missing SEQUENCE tag