standalone blast error, stuck between two errors, i.e; query not accessible file error and BLAST Database error: No alias or index file found for protein database

Question

[Priya]

STANDALONE BLAST is already installed in my remote server, and i am trying to do a simple blast between my protein query sequence and a file of whole protein sequences present in an organism. i made a database fold and made database with command-

makeblastdb -in GCF_000240185.1_ASM24018v2_protein.faa  -dbtype prot

then executed blastp comman-

blastp -query ~/firstref_seq -db /home/priya/BLAST/database/GCA_900635735.1_32875_B01_protein.faa -out test_results.txt

but its showing error-

BLAST Database error: No alias or index file found for protein database [/home/priya/BLAST/database/GCA_900635735.1_32875_B01_protein.faa] in search path [/home/priya/BLAST/database::]

i am executing blastp command in same directory where my database is present. someone tell me what's the problem here?

[cpad0112]

errors are clear. Make sure that files and index is available on remote machine, for program to run.

Answer 1

[Mensur Dlakic]

Computers have no sense of humor. When you get a message that a file is not there, your computer is not kidding. You can convince yourself, and maybe help us help you, by typing this command and letting us know the output:

ls -al /home/priya/BLAST/database/GCA_900635735.1_32875_B01_protein.faa*

It keeps things tidier if you keep your thread titles shorter, maybe something like BLASTP Database error.