seurat dotplot issue

Question

[clizama]

Hi,

I did some graph Dotplots from a dataset: I did the dotplot in two different ways but does not have too much sense the result:

The first I group by CellType and split by Control and treatment: The output looks like the Treatment is higher. https://user-images.githubusercontent.com/18195075/154424811-ad9f1d08-b958-4bea-84b3-9f22489ba55e.jpg The Second I just group.by Control and treatment (This is the average of all celltype). But the result is the opposite?? https://user-images.githubusercontent.com/18195075/154425033-f9310ce3-71b6-4f99-bbab-133125b8dfbd.jpg Do you know if I missing something??

[abedkurdi10]

Could you please share with us the commands you used to generate those two plots?

Answer 1

[clizama]

Plot1 Dotplot(object, features = c("MRC1"), group.by = "CellType", split.by = "orig.ident")

Plot2 Dotplot(object, features = c("MRC1"), split.by = "orig.ident")

[clizama]

I escalated the percent express cells and now I can see the dot.