Is there a tool available to check a Tm for a DNA fragment or sequence?

Question

[mmghaffar29]

Is there a tool available to check a Tm for a DNA fragment or sequence?

Answer 1

[Shred]

There's multiple software out there. I'd suggest this, written in R. https://aravind-j.github.io/rmelting/reference/melting.html

Based on SantaLucia [1] (as mostly of the software out there)

[1] https://www.pnas.org/content/95/4/1460

Answer 2

[benformatics]

If you are doing this manually then a lot of wet lab people use: https://www.thermofisher.com/ch/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html