Entering edit mode
2.7 years ago
poecile.pal
▴
50
Good morning,
Using hisat2, I mapped reads on transcripts_longestiso_longorfs.fasta
(de novo transcriptome assembly of non-model organism) and obtained .sam file. Using samtools, I converted it to .bam, sorted and indexed:
samtools view -bS hisat2_map_learn.sam > hisat2_map_learn.bam
samtools sort hisat2_map_learn.bam hisat2_map_learn_sorted
samtools index hisat2_map_learn_sorted.bam
Please tell me where can I find the verified workflow of using GATK to search for SNP in my case?
Best regards, Poecile