Question

I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help

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2.7 years ago

mibtisamnasar ▴ 10

I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help in this.

sequencing illuminasequencing ngs illumina dataanalysis • 789 views

ADD COMMENT • link updated 2.7 years ago by swbarnes2 14k • written 2.7 years ago by mibtisamnasar ▴ 10

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It's not a pushbutton thing you are asking. Have you googled a variant calling tutorial?

ADD REPLY • link 2.7 years ago by swbarnes2 14k

score 0 · Answer 1 · 2022-03-08

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2.7 years ago

abedkurdi10 ▴ 190

You can use velvet or spades for reads assembly, then use blast on the obtained FASTA to know to which bacteria your samples resemble.

ADD COMMENT • link 2.7 years ago by abedkurdi10 ▴ 190