Metagenomic taxonomic assignment for beginner
1
0
Entering edit mode
2.7 years ago
seal • 0

Hello, a little bit about myself. I'm a first year bioinformatic grad student who somehow got himself into a mock metagenomic project (there will be an actual project in half a year). I have no prior experience with metagenomics side and there are no courses being offered in my university that covers the basic of metagenomic.

For the project, I am expected to 1) find and download human metagenomic data (raw reads), 2) perform taxonomic assignment (taxonomic profiling, etc), and 3) perform comparative metagenomic is possible.

I somehow managed to download files (in fastq.gz format) from the human microbiome project database. I vaguely know I should use software such as Kraken but I honestly have no clue (and no one to ask in rl) who should I even begin. If anyone can point me towards the right direction or leave hints, it will be a life saver.

metagenomics • 762 views
ADD COMMENT
3
Entering edit mode
2.7 years ago

Read some papers

  • 2017 Shotgun metagenomics, from sampling to analysis
  • 2017 Critical Assessment of Metagenome Interpretation—a benchmark of metagenomics software
  • 2019 Benchmarking Metagenomics Tools for Taxonomic Classification.pdf
  • 2020 LEMMI: a continuous benchmarking platform for metagenomics classifiers
  • 2021 Critical Assessment of Metagenome Interpretation - the second round of challenges
ADD COMMENT
0
Entering edit mode

Thank you so much

ADD REPLY

Login before adding your answer.

Traffic: 2288 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6