Metagenomic taxonomic assignment for beginner
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2.7 years ago
seal • 0

Hello, a little bit about myself. I'm a first year bioinformatic grad student who somehow got himself into a mock metagenomic project (there will be an actual project in half a year). I have no prior experience with metagenomics side and there are no courses being offered in my university that covers the basic of metagenomic.

For the project, I am expected to 1) find and download human metagenomic data (raw reads), 2) perform taxonomic assignment (taxonomic profiling, etc), and 3) perform comparative metagenomic is possible.

I somehow managed to download files (in fastq.gz format) from the human microbiome project database. I vaguely know I should use software such as Kraken but I honestly have no clue (and no one to ask in rl) who should I even begin. If anyone can point me towards the right direction or leave hints, it will be a life saver.

metagenomics • 760 views
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Entering edit mode
2.7 years ago

Read some papers

  • 2017 Shotgun metagenomics, from sampling to analysis
  • 2017 Critical Assessment of Metagenome Interpretation—a benchmark of metagenomics software
  • 2019 Benchmarking Metagenomics Tools for Taxonomic Classification.pdf
  • 2020 LEMMI: a continuous benchmarking platform for metagenomics classifiers
  • 2021 Critical Assessment of Metagenome Interpretation - the second round of challenges
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Thank you so much

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