How to change the score of reads to be negative on the antisense chain of the genome from bam file
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2.7 years ago
17318598206 ▴ 20

hello everyone, i have chip-seq dada, i have got the BAM file by mapping, then i extracted and compared reads on the forward and reverse strands,

now i want to change the score of reads to be negative on the reverse strand from bam file, so that allows me to visualize the peak in different directions,like this: https://data.cyverse.org/dav-anon/iplant/home/xucaoling/forward_reverse.png

so, how to make it?

thank you!

reverse score strand negative chip-seq • 406 views
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