Free software to view Chromatograms (Sanger Sequencing)

Question

[mathilde.thierry]

Hello,

I would like to find a free or inexpensive software to observe .ab1 files.

For the moment we use ChromasPro to align the sequences and then by doing Ctr+F we search for the variant that interests us, and depending on the base, we determine the genotype, and that for several patients and several variants at the same time. So we waste time doing it. We would therefore need software that tells us directly if the pattern (the mutation plus the bases that surround it) is present and should be able to keep in memory the patterns that interest us to find them quickly.

If anyone has an idea, I'm all ears. I already tried Ugene but I couldn't create a workflow that allowed to get if the pattern was present or not.

Thank you

Answer 1

[GenoMax]

tracy (LINK) is command line but you can also try https://www.gear-genomics.com/teal/ from same group.

novoSNP (LINK) is another option.

[mathilde.thierry]

NovoSNP is actually not too bad, we can quickly find the variant that interests us, thank you for your help !!