Entering edit mode
2.7 years ago
SushiRoll
▴
140
Hi everyone,
I am using Kraken on a set of Illumina reads from a metagenomics project. In the past I have used dada2 for 16S amplicons and had to deal with chimera filtering. My question is the following, does a chimera removal step need to be performed before running Kraken or does Kraken manage the chimeras by itself? If I have to filter chimeric sequences, could anyone provide me a pipeline that gives me a Kraken compatible output?
I have tried reading about this but I am not able to find anything about it in the Kraken manual.
Thank you very much in advance.
Alan.