May I run scRNA-seq data in Seurat without barcode file?

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2.7 years ago

mogollon.garcia • 0

Hi!, I'm working with the dataset GSE121861, but its don't have a barcode file. May I run this data in Seurat without this file?. Thanks for the comments.

scRNA-seq • 716 views

ADD COMMENT • link updated 2.7 years ago by GenoMax 147k • written 2.7 years ago by mogollon.garcia • 0

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I don't know that anyone wants to download those files to see what's going on with them; you might get more of a response if you show the top few lines of ever file you've got.

ADD REPLY • link 2.7 years ago by swbarnes2 14k

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File 1. Column data. Few lines:

Sample_ID   Model   Protocol    Experiment
AD3950-S1   CT26    fresh   EXP-17-AD3950
AD3950-S1   CT26    fresh   EXP-17-AD3950
AD3950-S1   CT26    fresh   EXP-17-AD3950

File 2. Row data Few lines:

GeneSymbol  ENSMUSG_ID
Xkr4    ENSMUSG00000051951
Gm1992  ENSMUSG00000089699
Gm37381 ENSMUSG00000102343
Rp1 ENSMUSG00000025900

File 3. Expression data. It is the matrix with the expression values.

ADD REPLY • link updated 2.7 years ago by GenoMax 147k • written 2.7 years ago by mogollon.garcia • 0

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