off-target predictions to prioritize list of sgRNA designs
0
0
Entering edit mode
2.8 years ago
Anand Rao ▴ 640

Greetings!

Is there a tutorial / manual for the command line version of CHOPCHOP that explains

  1. how to design sgRNAs for non-human genome (not available on their web version)
  2. predict off-target(s)
  3. select best performing sgRNAs for each target locus

There are some resources available at

  1. https://bitbucket.org/valenlab/chopchop/src/master/README.md, and

  2. discussion threads under issues at https://bitbucket.org/valenlab/chopchop/issues

Do users here on BioStars know of any additional resources - like an actual tutorial perhaps?

Thanks in advance

sgRNA CRISPR CHOPCHOP • 433 views
ADD COMMENT

Login before adding your answer.

Traffic: 2134 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6