Gene ontology in the Microarray data

Question

[Mostafa]

Hi dears

I have analysed the microarray data of Affymetrix from Hordeum vulgare by R software

I have obtained the probe set IDs and know have Gene Ontology (Description), How can i do this work?

Kind regards, Mostafa

[Kevin Blighe]

Hi Mostafa, can you share an example of the data that you currently have? Kind regards, Kevin.

[Mostafa]

Hi dear

These are microarray ProbeStet IDs from Affymetrix (From Hordeum vulgare) I want to know Gene Ontology of these ProbeStet IDs Very thanks

Contig21426_at
Contig1718_s_at
Contig24415_at
Contig1762_s_at
Contig12748_at
Contig3112_at
Contig15682_at
Contig4281_s_at
Contig2768_s_at
Contig7003_at
Contig11477_at
Contig15186_at
HT11N18r_s_at
Contig13704_at
Contig2714_at
HV12A05u_s_at
rbasd2c09_s_at
Contig13161_at
EBem09_SQ001_O02_s_at
Contig8641_at
Contig11241_at
HS01M21w_s_at
Contig17178_at
Contig15773_at
HVSMEf0019G08r2_s_at
Contig981_s_at
HVSMEb0010E16r2_s_at
Contig6043_at
Contig2416_at
Contig2716_s_at
Contig4545_s_at
Contig4958_at
Contig4544_s_at
Contig12125_at
Contig422_at
Contig3111_at
Contig7254_s_at
Contig13073_at
Contig10206_s_at
Contig5126_at
rbaal9h21_s_at
Contig4281_at
Contig4205_at
HVSMEf0010N18r2_s_at
Contig18582_at
Contig5487_s_at
Contig23673_at
Contig4751_at
HV06O23u_at
Contig11328_at
HVSMEi0013J13r2_at
Contig6505_at
Contig12291_at
Contig7437_at
Contig5929_at
Contig4954_s_at
Contig9265_at
Contig594_x_at
EBro08_SQ008_K12_at
Contig3315_s_at
Contig4955_s_at
Contig8078_at
Contig19393_at
HVSMEn0007B19f_s_at
Contig2403_at
Contig7603_at
Contig16368_at
Contig12400_at
Contig17968_at
Contig1765_at
Contig12070_at
Contig11799_at
Contig418_at
Contig11173_at
Contig13906_at
Contig12281_at
Contig11611_at
Contig8087_at
Contig3113_at
Contig11213_at
Contig5196_at
Contig3552_s_at
Contig9774_s_at
Contig6541_at
Contig14279_at
Contig13376_at
Contig18583_at
EBro07_SQ001_A12_at
Contig17135_s_at
Contig18447_at
Contig2147_at
Contig1776_s_at
HVSMEf0014C12r2_s_at
Contig12550_at
Contig13990_at
Contig6623_s_at
Contig23531_at
HV12E23u_at
HVSMEf0023D17f_s_at
Contig4830_at

[Kevin Blighe]

You checked the NetAffx files? - see our previous thread Microarray

[Mostafa]

Hi dear Yes i checked and done your advise, but i could not solve my problem

I need to Gene Ontology from ProbeSet IDs

Thanks Mostafa

[Kevin Blighe]

agriGO (https://academic.oup.com/nar/article/45/W1/W122/3796337) had data for this species, however, the database is no longer available.

Did you try the solution here: https://support.bioconductor.org/p/131067/#131118 ?

[Mostafa]

hi dear

Unfortunately, i couldn't solve this problem (Taking Gene Ontology) in my thesis

The GPL is GPL1340. How can i solve this problem in R software ? Is there method in R?

kind regards