How to create correct adapters for trimming (with trimmomtaic)

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2.8 years ago

Easybella • 0

Dear community,

I am using Trimmomatic (Trimmomatic-0.36) to trimm Illumina reads from a transcriptome data set.

When I ask the company to provide me with the adapters needed for trimming, I get the following list:

Read1:

Read2:

These reads I could now in general use and write them to an adapters list that I feed into trimmomatic.

But, how do I know if I have to also give the reverse complements?

I would be very greatful for your help and some insight!

thank you!

sequencing trimmomatic RNA adapters trimming • 537 views

ADD COMMENT • link updated 2.8 years ago by lieven.sterck 15k • written 2.8 years ago by Easybella • 0