-p argument in freebayes for pooled sample

Question

[taniamahmood38]

Dear all,

I am making the vcf file using freebayes of my pooled-seq data of 12 individuals. I am confused that in -p argument should I write 12 (which is number of individuals) or 24 (which is the number of haplotypes in my pool)? Kindly guide me.

Thanks in advance