Hello all,
I am having some trouble with interpretation of enrichment scores (ES). I am beginning a project identifying drugs for drug repositioning for a medical condition. I am using a program/tool that ranks small molecules according to their ability to dysregulate an input set of pathways. The program uses a method analogous to GSEA to accomplish this. Now, perhaps I am naive and do not understand the intricacies of the calculations or their meaning, but I am having trouble understanding what the enrichment score exactly means?
To my understanding the enrichment score of a drug coincides with its ability to dysregulate or impact the pathway of interest, however I am confused as to how to interpret a positive or negative value. Does a negative or positive value indicate down-regulation and up-regulation respectively? Thank you all in advance for your time reading this and any help you can provide!
What values are you using as input to this program to calculate ES? Is it the log fold changes from RNA-seq differential expression?
Hi,
First off thank you for your response. I am using the program Gene2Drug, there are no log fold changes from RNA-seq expression that I enter into the program. Using the program, you enter in Pathways of Interest or Genes and it will return a list of drugs.