I have several annotated bacterial genomes that I have been analyzing. I have run them through the web-browser utility BlastKOALA to get KEGG functional annotations. This generates a list of KO numbers and allows exploration of various pathways and maps, however, I am trying to find a way to summarize the functional potential of the overall genome.
Specifically, the results from the tool show a summary page that includes a piechart showing the distrubition of functional categories from the annotation, which is basically what I want. However the data summarized at this point is not provided or accessible in this form to the user. If I explore what is available to me, it is all at a much greater resolution. For example, I want to know how many genes are associated/categorized as "amino acid metabolism" however the resolution of the data I am provided would be "K01079 serB, PSPH; phosphoserine phosphatase [EC:3.1.3.3]" and would require me to manually curate it all to a lower resolution before I can summarize.
Can anyone provide me some insight into other approaches/tools?
do you have a list of genes of interest?