Entering edit mode
2.6 years ago
isha.lily20
▴
10
Hello Researchers
Teacher gave work to do transcritpome annotation with only transcriptome annotation GTF file which was obtained after cufflinks of 2 expression data control and test, the GTF file only contains exon.
How to find all annotation from these after cufflinks GTF file
promoter/cds?
Work I tried already : from reference genome GTF file I separated all annotated regions and extracted sequences than did blastn with transcriptome sequences and took top most hit, but teacher told focus on only transcriptome sequences for annotation, I am confused, please help me.
Thank you
The idea of assignments is to figure it out yourself. At least present some outline of ideas.