How to bin groups of 20 cells based amount of a metadata value
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2.6 years ago
cook.675 ▴ 230

I have a scRNAseq Seurat object that is virus infected cells. I have a metadata label that is a sum of the total viral transcripts in each cell.

Object$total.virus.count

I want to make groups of 20 cells based on the highest expression of viral transcripts. For example, if I were to sort based on total.virus.count, group 1 would be the first 20 cells. Group 2 would be the second 20 cells, etc... I would like to label these groups of 20 as another metadata column ("VirusGroup" = 1,2,3,4,5) so they can be subsetted and analyzed.

I have no idea how to do this but I tried order function and some dplyr calls but I have no been successful.

Any help would be greatly appreciated!

Thanks

data seurat scRNA-seq R • 574 views
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2.6 years ago
cook.675 ▴ 230

I solved it thanks!

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