Calculate a FACS channel's histogram
0
0
Entering edit mode
2.7 years ago
agout • 0

Hi there,

I'm not sure if this is the right place for this question, but I feel like not seeing the tree in the forest. ;) I have some data from a FACS experiment and want to plot histograms of a given channel.

The channel's data is an 1D array of decimals ranging from slightly below zero to 10^5. No matter if I use Excel or Python pandas, the histograms show that the majority of values is below 100 (counts > 3.5e5 of 2e4). The rare events with high signals are barely visualized. However, if I use Flowing Software 2 or look at the report of the facility (they're using BD Diva), the histograms only counts up to ~200 with most values above 100. Obviously, there's some data transformation going on. The axis in the dedicated FACS software seem to be log-scale (or logicle) and there are counts around zero, which are heavily compressed in the final graph. However, simply plotting the data on a log-scaled axis in Python does not help.

As said, not sure, whether this is the right place, because it's a more question about theoretical and mathematical background. ;) But if someone can give some explanations for a naive person, this would be great.

pandas FACS Python histogram • 722 views
ADD COMMENT
0
Entering edit mode

Can you post the python and the software plots, to get an idea?

ADD REPLY
0
Entering edit mode

They look like this:

Histogram plotted by FACS software

Histogram plotted with Matplotlib

ADD REPLY

Login before adding your answer.

Traffic: 1799 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6