Burkholderia assembly
0
0
Entering edit mode
2.6 years ago

Hello there, Recently I am working with Burkholderia spp. As it has 2 chromosomes, is it possible to get individual fasta files for each chromosome? If possible, which assembler should I use? I have used Spades for other assemblies which have single chromosome. The sequencing platform was illumina miseq.

assembly Burkholderia • 730 views
ADD COMMENT
1
Entering edit mode

is it possible to get individual fasta files for each chromosome?

With only Illumina sequencing data is very unlikely; no matter which assembler you use

ADD REPLY
0
Entering edit mode

We have a plan do Minion sequencing by next week. Will it be possible then?

ADD REPLY
1
Entering edit mode

It could be possible. In that case, try Unicycler

ADD REPLY

Login before adding your answer.

Traffic: 2182 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6