Question

featurecounts index bai file

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2.8 years ago

biology_inform ▴ 50

Is indexed bam file (.bai) file required for featureCounts? In HTSeq-counts it was required.

featurecounts rna-seq • 1.0k views

ADD COMMENT • link updated 2.8 years ago by ATpoint 86k • written 2.8 years ago by biology_inform ▴ 50

score 1 · Answer 1 · 2022-04-21

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Entering edit mode

2.8 years ago

ATpoint 86k

No. Please see the manual, it instructs at no point to index the BAM file which makes sense because for paired-end data it anyway requires grouping by name and indexing requires grouping by coordinate.

ADD COMMENT • link 2.8 years ago by ATpoint 86k