Paired-end dataset from SRA database
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Entering edit mode
2.7 years ago

I downloaded the paired-end SRP325386 dataset from SRA using the following command:

esearch -db sra -query SRP325386 | efetch -format runinfo | cut -d ',' -f 1 | grep SRR | xargs -n 1 ./../sratoolkit.3.0.0-ubuntu64/bin/fastq-dump --split-3 --gzip --skip-technical --readids -W --read-filter pass  

However, the runs that were downloaded were not split into two files. For each run, I only get one file each SRR*.fastq.

How do I get the paired ends in two separate files?

paired-end sra • 613 views
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Entering edit mode
2.7 years ago
ATpoint 86k

It's not paired-end, nor Illumina, it's PacBio so one file is expected. https://www.ncbi.nlm.nih.gov/sra/SRX13003932[accn]

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