Entering edit mode
2.6 years ago
Thomas
•
0
I have a circular plasmid about 8 thousand in size. I exposed it to Hind and PstI, together and separately. The resulting fragments are Hind 4000, 2500, 1000, 500. Pst 5000, 2200, 750. Hind+Pst 3500, 2200, 1000, 700, 500, 250. We need to build a restriction map. are there any programs to do this?
I guess you are looking for something like this: https://doi.org/10.1006/geno.1996.0215
In the past we used to put these together as one would a jigsaw puzzle using a pen/paper. There must be programs available to do this automatically though I am not immediately aware of any. Someone should be along with a useful answer.
BTW: Is this a homework question? If it is you need to show some effort on your part to find a solution.